EAP4EMSIG -- Enhancing Event-Driven Microscopy for Microfluidic Single-Cell Analysis
Nils Friederich, Angelo Jovin Yamachui Sitcheu, Annika Nassal, Erenus Yildiz, Matthias Pesch, Maximilian Beichter, Lukas Scholtes, Bahar Akbaba, Thomas Lautenschlager, Oliver Neumann, Dietrich Kohlheyer, Hanno Scharr, Johannes Seiffarth, Katharina Nöh, Ralf Mikut
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Abstract
Microfluidic Live-Cell Imaging yields data on microbial cell factories. However, continuous acquisition is challenging as high-throughput experiments often lack realtime insights, delaying responses to stochastic events. We introduce three components in the Experiment Automation Pipeline for Event-Driven Microscopy to Smart Microfluidic Single-Cell Analysis: a fast, accurate Deep Learning autofocusing method predicting the focus offset, an evaluation of real-time segmentation methods and a realtime data analysis dashboard. Our autofocusing achieves a Mean Absolute Error of 0.0226 m with inference times below 50~ms. Among eleven Deep Learning segmentation methods, Cellpose~3 reached a Panoptic Quality of 93.58\%, while a distance-based method is fastest (121~ms, Panoptic Quality 93.02\%). All six Deep Learning Foundation Models were unsuitable for real-time segmentation.